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[2003] 7. Yong-Ji Zhang, Ki-Beom Kim, Mi-Jin Kim, Jung-Bin Lee |
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Poster :
Date : 09-12-21 19:28
Hit : 1013
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Publication; issue : 2003 Year 27 Vol 1 iss 45 p
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(207.9K), Down : 35, 2009-12-21 19:28:45 | |
Analysis of Polymorphism of Penta E and Penta D in Korean.
Korean J Leg Med. 2003 May;27(1):45-50. Korean.
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Department of Forensic Medicine, Seoul National University College of Medicine, Korea.
A duplex PCR reactions were designed for two pentameric (Penta D, Penta E) short tandem repeats (STR) loci to study the allele frequencies and the applicability of genetic variation in these loci to forensic case works. For 323 unrelated Koreans DNA was extracted using Qiagen DNA blood kit. The fluorescently labeled amplified products were analyzed by 8M urea, 5% long range gel electrophoresis in ABI-377 sequencer and followed data analysis by Genescan Analysis and Genotyper DNA fragment analysis software. The PD, PIC and PE were above 0.92, 0.76 and 0.66, respectively. No deviation from Hardy-Weinberg equilibrium was observed of Penta D locus (p=0.35, observed number>1).
Key Words : Pentameric, STR, Penta D, Penta E, Korean
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