HOME사이트맵Eng
학회소개 학회일정 학회지 토론방 게시판 자료실 관련사이트


  학회지  
     학회지 소개 
     편집위원 
     논문투고규정 
     학회지 검색 


학회지 검색
    > 학회지 검색 >>  2009
  [2009] 12. Hye Yeon Kim, Byoung Su Jang, Ai Hua Zhang, Seung Bum Seo, Hye Yeong Lee, Soong Deok Lee
  Poster :      Date : 09-12-12 18:54     Hit : 2097    
  Publication; issue :  2009 Year  33 Vol  2 iss  153 p
   (947.7K), Down : 136, 2009-12-12 18:54:22
Species Specificity of a Commercial AmpFℓSTR® MiniFilerTM PCR Amplification Kit
Korean J Leg Med. 2009 Nov;33(2):153-158. Korean.
 

Department of Forensic Medicine and Institute of Forensic Medicine, Seoul National University College of Medicine, Seoul, Korea. sdlee@snu.ac.kr


Recently forensic scientists have focused on extending the applicability of STR to degraded or tiny amount DNA. Short amplicon may be one of the solutions to these samples and several commercial kits are available for this purpose. Before practical casework, validation is necessary for newly introduced kit. We tried to check how newly introduced STR kit, AmpF
STR
® MiniFilerTM PCR Amplification Kit, reacts with some animal DNAs. We tried 27 animal DNA samples and checked whether the above kit amplify animal DNA, and next how the band appears on electropherogram. We compared the results with popular STR kits. With AmpFSTR® MiniFilerTM PCR Amplification Kit, we could get several bands on electropherogram for some species, but we could not designate proper allelic number compared to allelic ladder for most loci except the following loci, D13S317, D2S1338, D21S11, D18S51, FGA. The D18S51 locus was outstanding in that some species showed definite designated alleles, but the allelic number was not different depending on species. This was the same with another popular STR kit, AmpFSTR® IdentifilerTM PCR Amplification Kit, which was from the same company. The another kit from another company, PowerPlex® 16 System showed different phenomenon with more increased number of amplified bands which were usually differ on size when compared to allelic ladder.


Key words : Species Specificity, STR, Forensic, MiniFiler

Original article



   hit   datetime
Total 613
Category  iss Date / Subject / Contents / Writer
2020 (3) 6. Moonhee Jung, Su Jin Lee, Seong Ho Yoo2, Hyung-Seok Kim

Death from 2,4-Dinitrophenol Poisoning: An Autopsy Case

Korean J Leg Med 2020;44:140-142

2020 (3) 5. Milim Kim, Soong Deok Lee, Moon-Young Kim

Intentional Death Caused by the Overdose of Nonprescription Drugs: An Autopsy Case of Acetaminophen Poisoning

Korean J Leg Med 2020;44:134-139

2020 (3) 4. Young-Hoon Jo, Bo-Kyung Kong, Ji-Sook Min, Inseok Choi, J…

Two Cases of the Postmortem Testing of Ethyl Glucuronide and Beta-hydroxybutyrate for Chronic Alcoholism

Korean J Leg Med 2020;44:129-133

2020 (3) 3. Yun Seol Na, Ji Hye Park

Unusual Asphyxia: Two Autopsy Cases Report

Korean J Leg Med 2020;44:123-128

2020 (3) 2. Gi Yeong Huh

Progress in Method and Reliability of Diatom Test in Drowning

Korean J Leg Med 2020;44:115-122

2020 (3) 1. Youn Shin Kim, Tae Eun Kim

Proposal of Comprehensive Act for Postmortem Examination to Improve Death Certification System

Korean J Leg Med 2020;44:103-114

2020 (2) 6. Min Jee Park, Ock Kim, Hongil Ha

Death by Nitrite Intoxication: Report of 14 Cases

Korean J Leg Med 2020;44:96-101

2020 (2) 5. Yonghan Jung, Sangbum Lim, Youngsan Ko, Seonjung Jang, Hy…

Fatal Complications of Dental Implant Surgery: An Autopsy Case Report

Korean J Leg Med 2020;44:92-95

2020 (2) 4. Ji Eun Park, Su Jin Jeong, Sang Hyun Park, Sang Eon Shin,…

A Comparison of Growth Curve Models for Forensically Important Flies Using Sigmoid Functions

Korean J Leg Med 2020;44:84-91

2020 (2) 3. Jong-Pil Park, Bon Young Koo, Nak-Won Lee

Postmortem Diagnosis of Anaphylactic Shock by Histopathological Examination of Mast Cell

Korean J Leg Med 2020;44:76-83

 1  2  3  4  5  6  7  8  9  10    

24시간 동안 이 창을 다시 열지 않음
24시간 동안 이 창을 다시 열지 않음
24시간 동안 이 창을 다시 열지 않음